Advances in imaging technologies continue to transform our understanding of complex biological systems and the design of next-generation materials. This symposium will highlight the critical role of chemistry in developing and applying imaging techniques across diverse fields, from visualizing molecular dynamics in live cells to mapping nanoscale features in semiconductors. Topics will include the design of novel chemical probes and contrast agents, innovations in spectroscopic and microscopic imaging, and chemical strategies enabling high-resolution, multi-modal visualization. By bringing together experts in chemical biology, materials chemistry, and imaging science, this symposium aims to foster cross-disciplinary dialogue and spark new collaborations at the interface of chemistry and imaging.